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src family kinase sfk py416  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc src family kinase sfk py416
    Src Family Kinase Sfk Py416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+src+family+py416+antibody/Phospho-Src+Family+(Tyr416)+Antibody/pm20583816__cb100099h_si_001-12-10-15
    Average 96 stars, based on 2356 article reviews
    src family kinase sfk py416 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Abl-mediated PI3K activation regulates macrophage podosome formation.
    Article Snippet: Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.. Primary antibodies included anti-paxillin (612405, BD Biosciences Pharmingen; 1:1000, 1 day), anti-vinculin (V9264, Sigma; 1:100, 2 day), phosphospecific cAbl (Tyr488) antibody (1:100, 2 day), and phospho-Src family (pY416) antibody (Cell signaling, 2101, 1:500, 2 days).. After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.

    Article Title: Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays.
    Article Snippet: A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).. Src Antibody Sampler Kit containing Phospho-Src Family (pY416) antibody (no. 2101) for activated Src and Src (36D10) antibody (no. 2109) for cellular total Src were purchased from Cell Signaling Technology.. Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).

    Transformation Assay:

    Article Title: Abl-mediated PI3K activation regulates macrophage podosome formation.
    Article Snippet: Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.. Primary antibodies included anti-paxillin (612405, BD Biosciences Pharmingen; 1:1000, 1 day), anti-vinculin (V9264, Sigma; 1:100, 2 day), phosphospecific cAbl (Tyr488) antibody (1:100, 2 day), and phospho-Src family (pY416) antibody (Cell signaling, 2101, 1:500, 2 days).. After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.

    Article Title: Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays.
    Article Snippet: A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).. Src Antibody Sampler Kit containing Phospho-Src Family (pY416) antibody (no. 2101) for activated Src and Src (36D10) antibody (no. 2109) for cellular total Src were purchased from Cell Signaling Technology.. Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).

    Activity Assay:

    Article Title: Abl-mediated PI3K activation regulates macrophage podosome formation.
    Article Snippet: Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.. Primary antibodies included anti-paxillin (612405, BD Biosciences Pharmingen; 1:1000, 1 day), anti-vinculin (V9264, Sigma; 1:100, 2 day), phosphospecific cAbl (Tyr488) antibody (1:100, 2 day), and phospho-Src family (pY416) antibody (Cell signaling, 2101, 1:500, 2 days).. After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.

    Article Title: Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays.
    Article Snippet: A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).. Src Antibody Sampler Kit containing Phospho-Src Family (pY416) antibody (no. 2101) for activated Src and Src (36D10) antibody (no. 2109) for cellular total Src were purchased from Cell Signaling Technology.. Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).

    Activation Assay:

    Article Title: Abl-mediated PI3K activation regulates macrophage podosome formation.
    Article Snippet: Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.. Primary antibodies included anti-paxillin (612405, BD Biosciences Pharmingen; 1:1000, 1 day), anti-vinculin (V9264, Sigma; 1:100, 2 day), phosphospecific cAbl (Tyr488) antibody (1:100, 2 day), and phospho-Src family (pY416) antibody (Cell signaling, 2101, 1:500, 2 days).. After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.

    Article Title: Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays.
    Article Snippet: A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).. Src Antibody Sampler Kit containing Phospho-Src Family (pY416) antibody (no. 2101) for activated Src and Src (36D10) antibody (no. 2109) for cellular total Src were purchased from Cell Signaling Technology.. Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).

    Western Blot:

    Article Title: Abl-mediated PI3K activation regulates macrophage podosome formation.
    Article Snippet: Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.. Primary antibodies included anti-paxillin (612405, BD Biosciences Pharmingen; 1:1000, 1 day), anti-vinculin (V9264, Sigma; 1:100, 2 day), phosphospecific cAbl (Tyr488) antibody (1:100, 2 day), and phospho-Src family (pY416) antibody (Cell signaling, 2101, 1:500, 2 days).. After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.

    Article Title: Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays.
    Article Snippet: A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).. Src Antibody Sampler Kit containing Phospho-Src Family (pY416) antibody (no. 2101) for activated Src and Src (36D10) antibody (no. 2109) for cellular total Src were purchased from Cell Signaling Technology.. Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).

    Expressing:

    Article Title: Abl-mediated PI3K activation regulates macrophage podosome formation.
    Article Snippet: Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.Shortly after fixation, cells were permeabilized by TritonX-100 (0.1% in PBS, 10min, 37°C), passivated with the blocking buffer (10% donkey serum and 1% bovine serum albumin) at 4°C overnight, and then incubated with the primary antibody at 4°C.. Primary antibodies included anti-paxillin (612405, BD Biosciences Pharmingen; 1:1000, 1 day), anti-vinculin (V9264, Sigma; 1:100, 2 day), phosphospecific cAbl (Tyr488) antibody (1:100, 2 day), and phospho-Src family (pY416) antibody (Cell signaling, 2101, 1:500, 2 days).. After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.After being rinsed with 25 mL of PBS, cells were subsequently incubated with secondary antibodies in room temperature for 2 hours.

    Article Title: Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays.
    Article Snippet: A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).A rabbit polyclonal antibody against survivin (ab469) was purchased from Abcam (Cambridge, UK).. Src Antibody Sampler Kit containing Phospho-Src Family (pY416) antibody (no. 2101) for activated Src and Src (36D10) antibody (no. 2109) for cellular total Src were purchased from Cell Signaling Technology.. Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).Anti-avian Src, clone EC10 antibody (05–185) was purchased from Upstate (Lake Placid, NY, USA).



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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Blood flow patterns switch VEGFR2 activity through differential S-nitrosylation and S-oxidation

    doi: 10.1016/j.celrep.2023.113361

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: c-SRC (pY416) , Cell Signaling Technology , Cat# 2101; RRID:AB_331697.

    Techniques: Virus, Recombinant, Biotin Switch Assay, Mutagenesis, Control, Sequencing, Software